One important factor influencing lifespan and aging in Caenorhabditis elegans (C. elegans) is the insulin/IGF-1 signaling (IIS) pathway, which is controlled by the daf-2 gene. In C. elegans, environmental factors like temperature are known to significantly alter lifespan and stress responses, while RNA interference (RNAi) provides a reliable technique for examining gene function. With an emphasis on comprehending the interplay between genetic and environmental factors in aging, this study investigates the effects of R NAi-mediated knockdown of daf-2 on lifespan across four different temperature conditions (4°C, 10°C, 20°C, and 30°C). Synchronized populations of C. elegans were exposed to daf-2 RNAi and maintained under controlled temperature conditions to assess their lifespan. Although the study is ongoing and results have not yet been collected, it is hypothesized that daf-2 knockdown will result in reduced lifespan relative to controls, with the most pronounced effects occurring at temperature extremes (4°C and 30°C). This research seeks to elucidate the complex interplay between IIS signaling and environmental stressors in lifespan regulation, contributing to the broader understanding of genetic and environmental influences on aging and resilience mechanisms.
